产品详情介绍
本产品利用特殊的重组酶和同源重组的原理,可以将任意方法线性化后的载体和与其两端具有15-25 bp重叠区域的PCR片段定向重组,可以实现1-7个片段的高效无缝拼接。
• 快速:仅需要5~15分钟反应时间。
• 简单:不受片段酶切位点的影响,无需对片段酶切。
• 高效:阳性率达95% 以上。
• 无缝:不引入额外的序列。
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2 Shi C, Yang X, Hou Y, et al. USP15 promotes cGAS activation through deubiquitylation and liquid condensation[J]. Nucleic Acids Research, 2022(IF 14.90)
3 Jin Q, Yang X, Gou S, et al. Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression[J]. Science China Life Sciences, 2022.(IF 10.37)
4 Xu Y, Zhu T F. Mirror-image T7 transcription of chirally inverted ribosomal and functional RNAs[J]. Science, 2022.(IF 63.71)
5 Li B, Zhu L, Lu C, et al. circNDUFB2 inhibits non-small cell lung cancer progression via destabilizing IGF2BPs and activating anti-tumor immunity[J]. Nature communications, 2021.(IF 12.12)
6 Liu S, Fan L, Liu Z, et al. A Pd1–Ps–P1 feedback loop controls pubescence density in soybean[J]. Molecular plant, 2020.(IF 12.08)